How to deal with TRIzol aerosolization during FACS Sorting directly into TRIzol?

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Didier Poncet

I don't see why you would have aerosolization of trizol; just put some trizol in the collecting tube; your cells will fall down directely in the trizol an be lyzed immediatly....

Koji Yoshida

Didier Poncet Thank you for your comment. My concern is that aerosolization of TRIzol might occur when the sorted cells come into contact with it, due to the high-speed flow during the sorting process.