Koji Yoshida

3 Questions 11 Answers 0 Followers

Questions related from Koji Yoshida

How to deal with TRIzol aerosolization during FACS Sorting directly into TRIzol?

I am currently collecting 1k-100k cells through FACS sorting for qPCR and RNA-seq. Several online protocols suggest sorting cells directly into TRIzol, and I have found this method effective for...

16 January 2024 1,948 2 View

What do two lines in FSC-A and FSC-H plot indicate in flow cytometry?

I am studying lymphocytes in the spleen using flow cytometry and I am trying to discriminate doublets in the FSC-A and FSC-H plot. In my data, there are two lines gated as P1 and P2. How should I...

19 February 2023 6,285 7 View

How can I identify the domains of a protein whose 3D structure is predicted by AlphaFold?

I used AlphaFold to predict the structure of a protein that has not been well studied. I have a pdb file of the predicted structure. Now I would like to identify the domains of the protein using...

01 November 2021 1,502 12 View