Hi, I have a question about HEK001 cell line. Which kind of medium and condition should I use for the culture? Thank you.
I always follow ATCC references:
http://www.atcc.org/products/all/CRL-2404.aspx#culturemethod
This research question explores the complex chain of relationships of how the oral microecology system affects various aspects of oral health and further affects systemic health. The oral...
09 July 2024 3,908 0 View
I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...
01 June 2024 1,352 0 View
Please I would link to know my RG ID. Thanks
26 April 2023 6,856 0 View
I was requested to find measures for actual behavior, rather than behavioral intentions, to be used in online surveys. The problem is that, in my research, I compare Activism and Clicktivism and...
16 March 2023 6,646 2 View
Hello, I have started culturing P. tricornutum CCAP 1055/18 on an enriched seawater medium (see Villanova et al 2021). But I am experiencing some problems related to these agglomerates (you can...
29 January 2023 6,956 1 View
Good morning, I am proposing home biogas production solutions. They are containers in very resistant flexible material. The problem is that, when temperatures drop, bacteria have a hard time doing...
03 October 2022 7,562 6 View
Im guessing one of my horizons isnt a regular surface, anyone know how to change it? or what the error message means? using Petrel 2021
13 April 2022 8,421 1 View
Hi everyone. I am studying and culturing marine diatom Phaeodactylum tricornutum for my PhD project. To harvest microalgal biomass after culturing it I currently centrifuge it, even if I am about...
06 December 2021 6,620 2 View
I've read in other questions that some cultures of Chlorella are preserved in agar plates and in refrigerator. But I was wondering if there is need of light also in agar plates? I am culturing a...
14 November 2021 4,994 5 View
I am developing a survey about voluntary organisations' needs in the city of Milan, and I am struggling to find some literature concrning this topic to organise a literature reviwe unpon which I...
12 September 2021 3,890 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View