Hello! I am currently planing a project with aptamers. I want to know how to creat a pool from randomized oligoneucleotide sequences from one oligoneucleotide library. Is it possible?
Fatal error in MPI_Allreduce: Other MPI error, error stack: MPI_Allreduce(1628)......: MPI_Allreduce(sbuf=000002459254A180, rbuf=000002459F86A140, count=4851, MPI_DOUBLE_COMPLEX, MPI_SUM,...
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
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One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...
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Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have incorporated the dispersion effect for the OPBE functional by specifying the S6, SR6 and S8 parameters for GD3 in Gaussian G09 package. For this purpose, I have defined the environment...
05 August 2024 1,494 2 View
how is prion can cause disease
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Nothing
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Jump 2 is the 1st app scientifically developed to measure your jump height.
31 July 2024 8,194 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...
06 July 2024 9,852 1 View
Hello, I hope this message finds you well. I am currently planning to synthesize a long chain oligonucleotide using our oligonucleotide synthesizer. However, I find myself uncertain about the...
07 May 2024 7,460 1 View
Hello. I have try to immobilize the thoilated aptamer onto the gold surface such as Gold coated silicon wafer and the gold coated screen printed electrode. However, it is failed, after aptamer...
29 April 2024 4,054 1 View
What is the best way to obtain high-performance aptamers after performing sequence amplification by asymmetric PCR other than biotin and streptavidin?
17 April 2024 6,973 2 View
I used the autodock4 tool for docking the aptamer and the ligand. I prepared the DNA aptamer following the process: sequence -> predict the 2d structure by MFold -> predict the 3D structure...
31 March 2024 2,772 0 View
molecular docking, aptamer
27 March 2024 4,622 2 View