Microparticles are too small for hemicytometer. They are usually counted with flow cytometry.

What scale are these particles and what are they composed of? If they are less than 5um nominal diameter, it will be difficult and I agree with Yuliya that flow is a better option. If they are bead size around 5um or more, you can use a hemacytometer the same way you would use it with cells. Avoid too many (

Thanks for the answers Anthony and yulia.

I want to count bead sized particles . This is helpful. thankyou .

Still i have a doubt my stock soln of bead is is 1.4E7

and as per the calculation a large number is coming. (Average count for each large square) X (Total volume of bead stock) X (Dilution Factor, if any) X (10000) = (Total # of beads in the stock solution)

I want to clear is this divided by 10000.Could you please help me out.

I prepared 500 microlitre from stock solution in 30ml of PBS and got the average as 12.53.dilution factor is 60. any body please help me to find a clear answer as how many particles will be there after preparing this solution.

thanks in advance.