Rdf of atoms from a COM or point in lammps or VMD?

Hello all, as I understand there isn't any straight way to get an rdf of atoms from the center of mass or a coordinate of specific atoms in lammps. I wonder if anyone know any trick or way to get...

16 February 2021 2,517 2 View

Could anyone help me about my problem with getting current in armchair MOS2 nanoribbon in transiesta?

Dear siesta users I am using transiesta code to get the I-V characteristics of Armchair MoS2 nanoribbon as scattering (with 8 unit cells) and electrode material. All the calculations (electrode,...

12 October 2020 4,306 6 View

About use of Fluoranthene as standard in HPLC?

Dear all; I want to work on the ecotoxicological effect of PAH (fluoranthene) on different organism species.... But I can not find its analytical standard in my country, Can I use Flournthene 98%...

05 July 2020 2,736 7 View

What kind of dialysis cassette is needed for CaCl2 in silk fibroin dissolving?

Hi! Recently, I am working on silk fibroin extraction for biomedical application. I have some problem in details of extraction and dissolving of silk fibroin. For example, I don't know what kind...

26 June 2020 3,927 1 View

What kind of dialyses cassette is require for extraction of silk fibroin base on nature protocol 2011 (Attached in following)?

I have some question about the laboratory process of extraction silk fibroin from Bombyx mori base the Prof. Kaplan protocol. How much Silk cocoon need for 20 experiments? How dialyzes the final...

15 June 2020 8,694 1 View

Is there any dye for staining the axons?

Hello everyone, I was wondering if there is any chemicals dye that stain the axons. Thanks

20 February 2020 7,303 3 View

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I'm trying to collect milk from our knockout mice (at the end of the lactation stage) to isolate milk exosomes. But I have a hard time with milk collection from mice. I have used either pump or...

22 January 2020 4,527 1 View

How to design primers for gene variant or isoforms? Preferably by Allel ID?

I am trying to design some priers for RT-PCR. As my genes have many isoforms, so I have to design primer for conserved sequences. but the results aren't satisfactory.

17 March 2019 3,346 3 View

Why is it used in bioassays studies with essential oils for effect pesticidal from acetone solvent instead of DMSO?

In bioassay studies, the effect of essential oils is used as an insecticide from acetone, but is used to study the microbial activity of essential oils from DMSO solvents. Why?

12 March 2019 5,664 7 View

Which type of surface modification should be used to match metal and graphene?

graphene is the reinforcement in the metal matrix

26 February 2019 4,160 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View