What is the best stabilizer to prevent cell clumping for algae cell counting by hemocytometer?

01 November 2023 0 4K Report

Badges
Science topic

More Samaneh Torbati's questions See All

What is the correct way of calculating CO2 from private transportation and what is correct emission factor?

Hi, I have a question about emission factors for passenger cars based on the EMEP/EEA air pollutant emission inventory guidebook 2023, in the link that I used to calculate the CO2 emission per...

04 July 2024 1,766 2 View

What is the best book or guideline for fertilizer recommendations?

Would you please introduce a comprehensive book for fertilizer recommendation guidelines for agricultural and garden plants. In fact, I am looking for sources that express the amount of fertilizer...

25 June 2024 7,351 4 View

How can I disperse carbon nanotubes in ethanol?

I want to use ethanol as solvent. please explain me about every details of this method for CNTs desperation. Please explain me completely and accurately how can I disperse carbon nanotubes in...

08 May 2024 908 0 View

Hi every one. I need a relatively cheap antimicrobial agent, especially Staphylococcus aureus, resistant to methicillin, fda approve, non-toxic,...?

I need a relatively cheap antimicrobial agent, especially Staphylococcus aureus, resistant to methicillin, fda approve, non-toxic with aqueous solvent and without precipitation and turbidity...

29 April 2024 5,970 4 View

How can I purify and functionalize CNTs by sulfuric acid and nitric acid?

I need to purify and then functionalize the carbon nanotube to improve its properties. The functional group that I want to add to the carbon nanotube is carboxylic acid (COOH). I would like to...

05 April 2024 746 1 View

I made a water in oil in water emulsion. For having its FTIR spectrum, should I use it in its liquid form or I should dry it before FTIR test?

The operator of FTIR spectrometer says because of water and OH peak, you won't have a good result.

18 January 2024 4,794 3 View

What is the role of PVP (Polyvinylpyrrolidone), when we use it in phosphate buffer for antioxidant enzymes activity assays?

What is the role of PVP (Polyvinylpyrrolidone), when we use it in phosphate buffer for antioxidant enzymes activity assays? such as assay of SOD or POD activity in plant cell....

03 November 2023 7,026 3 View

Host elements in a non-equimolar high entropy alloys?

What parameters can determine the host element or elements in a non-equimolar high entropy alloy? Considering that the element or elements have a higher atomic percentage in the composition, can...

11 September 2023 2,658 0 View

Is Tween 20 as a hydrophilic emulsifier making a foam?

As a hydrophilic emulsifier it leads into a foam during using it as emulsifier in emulsion. While agitating with Ultraturax or even in hotplate stirrer it happnes. How you ever had such an...

29 July 2023 8,165 4 View

Using the results of one simulation in the next simulation in COMSOL?

Hello I have generated a number of ions in COMSOL inside a chamber through the Electrostatic and Charged Particle Tracing modules (the first part of the simulation). Now I am going to move the...

17 July 2023 8,912 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View