I have submitted my manuscript on mechanistic interaction of proteins with nanoparticles. As i consider nanoparticle as a single molecule and calculated its molecular weight accordingly and written concentration in micromolar terms. I got comment that "The nanoparticles are known to be insoluble in a solvent but dispersed with a certain suspension density hence they can’t be formed as micromolar or nanomolar concentrations rather they should be in microgram or mg per mL suspension". I am having little trouble to convert those micromolar concentrations to microgram or mg/ml.
For reference: Article Binding interactions of MoS2 quantum dots with hemoglobin an...
How they have written the concentration of MoS2 (stock-0.5 mg/ml) in terms of mg/ml in the Fluorescence quenching graph (Figure 6 A) when they added 10 microL each time in titration.
If you calculated the molecular weight of the nanoparticle aa if it were a single molecule, you can convert µM to mg/ml using that molecular weight.
For example, for a 10 µM "solution" of a nanoparticle with a "molecular mass" of 1,000,000 g/mole, the concentration in mg/ml is:
1 x 10-5 mole/L x 106 g/mole = 10 g/L = 10 mg/ml
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