Hi all,
I've done WGS using nanopore, and have been able to successfully see 4 circularized plasmids and a full circularized chromosome.
How do I know that these are the full plasmids without hybrid assembly?
Hi
there are lot of aligner, even on web, but you can use this one from EBI:
https://www.ebi.ac.uk/Tools/msa/muscle/
all the best
fred
https://cge.food.dtu.dk/services/PlasmidFinder/
You can try PlasmidFinder for plasmid typing.
Alternatively, you can annotate the plasmid and check for featured genes like conjugative encoding genes and plasmid replication gene.
For beginner researcher, authors are confused to submit a paper on conference or journal publication in the majority times. I would like to give insight opinions from it.
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