I have worked with the synthesis of CuO nanoparticles through biosynthetisis pathway. I had problems to concentrate the filtrate results because the filtrate contain aqueous solvent. The synthesis of the results filtrate, I will use for the analysis of antibacterial tests. My goal is the result of the filtrate to increase antibacterial activity. The result of filtrate from synthesis may be have a compound secondary metabolite from plant extract and CuO nanoparticles.