I Have Problem my antibacterial assay use method disk diffusion and I Have problem with my extracts plants which still contained solvent water, extract with Copper Oxide Nanoparticles which still contained water solvent, and my CuO Nanoparticle did n't dissolve in DMSO 1 %.
I'm working with biosynthesis CuO Nanoparticle with aqoueus plant extract. The Purpouse my reaserach is learning antibacterial activity. The antibacterial compound that i have been use for antibacterial assay with diffusion disk method is crude extract plant, extract with CuO nanoparticle, and CuO nanoparticle. From results of my research the Compound did n't show inhibition zone.
Hello..
DO you need to evaporate water from samples? If it is so, apply vacuum to the aqueous solutions.
Freeze drying is best suited for your application. You may try it, if you have access to it; else perform vacuum assisted evaporation using rotary evaporator.
Dear Mr Vivekanand Chatpalliwar, and Mr Rohit Upadhyay
Thank you for your kindness to solve my problem. I think use rotary evaparotator is the solution. BUt My laboratory assistant prohibit me use rotaray evaporator because the solvent is water so he conclude can't work.
Sir. I have some question about antibacterial assay disk diffusion. i have problem about my result of antibacterial assay. my sample didn't show inhibiton zone.Can You help me to best procedure antibacterial activity?..
Thank You
To remove water, you should dissolve your extract in absolute ethanol and then pass the ethanol over sodium sulfate anhydrous to remove traces of water, then you can easily evaporate ethanol by rotary.
You can dissolve your extract in absolute ethanol and pass the ethanol solution over anhydrous sodium sulfate to remove water; then you can evaporate ethanol to get your extract void of water.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Pharmaceutical Botany
- Plant Extracts