Speedvac will definitely work....the only deciding thing will be whether you have the DNA solution in water or buffer......if it is buffer concentrating in a speedvac will reduce the volume but increase the buffer components and they may interfere in downstream reactions....
You could simply Ethanol precipitate it. Add 1/10th volume of 3M NaAc pH 5.2 and 3 volumes of 96% EthOH, vortex and incubate at -20 for about 20 minutes. Centrifute 20 minutes, wash the pellet with 70% EtOH and then resuspend in 1/10th the original volume. Alternatively you can use silica columns but the yield varies a lot with these columns
Yes, Sander, I understand, but I do not want to go through the ethanol precipitation simply because our DNA is too long (~ 150 kb), so an extra step of precipitation might break some chains. So can you suggest some gentler method? Thanks a lot!
quick question. can this be an easy solution? original solution-->cap open, in -20C, until water sublimation. then add less water/solvent. As long as using water rather TE, should this be fine?
Speedvac will definitely work....the only deciding thing will be whether you have the DNA solution in water or buffer......if it is buffer concentrating in a speedvac will reduce the volume but increase the buffer components and they may interfere in downstream reactions....