Yanfei Li

7 Questions 10 Answers 0 Followers

Questions related from Yanfei Li

Separation and purification of DNA isoforms

I have a sample of long chain DNA (~ 100 kb), and it is a mixture of different isoforms (supercoil, relaxed circular, nicked circular, and linear). But I only want relaxed circular from the...

28 December 2012 653 1 View

How to turn a regular microscope into a polarizing microscope?

I have a regular microscope and I want to study the liquid crystal phase of DNA solution, so I actually need a polarizing microscopy. I wonder what components should I add to my regular...

18 November 2012 4,717 16 View

How to concentrate DNA solutions?

I am having some DNA solution of 1 mg/ml, and I want to concentrate the solution to 10 mg/ml?

11 November 2012 8,664 10 View

How to achieve a theta condition for DNA solution, for the purpose of rheology?

I want to do some rheology on DNA at theta condition. But I do not know how to achieve the theta condition for DNA solutions. Under different NaCl concentrations, I am thinking to use static light...

11 November 2012 2,265 4 View

How to quantify each band in gel electrophoresis?

I am doing DNA electrophoresis. I wonder is there a way to quantify the amount of DNA in each band

24 October 2012 5,014 26 View

DNA stuck in wells during gel electrophoresis

and a little linear isoform. I run a gel on it, but I saw a bright sharp band stuck in the wells. Then I put

18 October 2012 4,358 4 View

DNA disappears after dialysis, does anyone know what could be wrong?

I have a DNA/TE buffer solution, the molecular weight of the DNA is 185 kb and this solution has some lipids and protein in it (from lysis of E-coli). The dialysis tubing I used is 300,000 MWCO. I...

15 October 2012 9,041 11 View