11 Questions 17 Answers 0 Followers
Questions related from Yanfei Li
I believe using alkaline lysis method to extract plasmid DNA from E. coli cells is a pretty mature method. To do it, I followed the popular protocol by adding 0.2 M NaOH and 1% SDS to DI water....
09 May 2014 1,949 1 View
I have a sample of long chain DNA (~ 100 kb), and it is a mixture of different isoforms (supercoil, relaxed circular, nicked circular, and linear). But I only want relaxed circular from the...
28 December 2012 698 1 View
I have a regular microscope and I want to study the liquid crystal phase of DNA solution, so I actually need a polarizing microscopy. I wonder what components should I add to my regular...
18 November 2012 4,737 16 View
I am having some DNA solution of 1 mg/ml, and I want to concentrate the solution to 10 mg/ml?
11 November 2012 8,711 10 View
I want to do some rheology on DNA at theta condition. But I do not know how to achieve the theta condition for DNA solutions. Under different NaCl concentrations, I am thinking to use static light...
11 November 2012 2,305 4 View
I am using AR-G2 to test some DNA solution, and the geometry I am using is a pair of 8 mm disposable aluminum plates. On the "Instrument Status" window, torque and shear rate cannot be zero...
27 October 2012 6,064 6 View
I am doing DNA electrophoresis. I wonder is there a way to quantify the amount of DNA in each band
24 October 2012 5,053 26 View
I have some 1,4-polybutadiene sample bought 5 months ago, and I put 0.2% of butylated hydroxytoluene (an anti-oxidant) in the sample to prevent degradation. The sample was kept in air and 4 degree...
22 October 2012 7,097 5 View
and a little linear isoform. I run a gel on it, but I saw a bright sharp band stuck in the wells. Then I put
18 October 2012 4,395 4 View
I have a DNA/TE buffer solution, the molecular weight of the DNA is 185 kb and this solution has some lipids and protein in it (from lysis of E-coli). The dialysis tubing I used is 300,000 MWCO. I...
15 October 2012 9,060 11 View
I have a DNA mixture, the largest of which is 185 kbp. I want to use pulsed field gel electrophoresis to analyze this mixture, but I cannot find a detailed protocol regarding the PFGE setting...
09 October 2012 9,990 5 View
