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Questions related from Tommy Zhou
I have to run some RNA samples from 0.5X TBE (tris-boric acid-edta) native gel (non-reducing condition). I have consistently experienced smearing associated with RNA bands. Also the smear appears...
21 April 2017 977 3 View
1\ small protein is in 5% polyethylene glycol solution 2\ can I use larger volume (like 3-4X) of ethanol or other solvent to precipitate protein from PEG solution? would PEG co-precipitate from...
22 November 2016 3,770 3 View
I've been working with RNA agarose gel electrophoresis and having trouble to visualize positive RNA control samples(1ug) and RNA ladder(3ug). enclosed is the best gel image I've got (please ignore...
28 May 2015 1,813 5 View
I am doing this experiment now: agrobacteria carry a viral vector with a EGFP signal, and I tried to figure out the ratio of cells that are infected (have EGFP).The plant I used is N. benthamiana....
22 October 2013 1,065 1 View
Came across a problem I never had before: when I use transmission electron microscope to visualize some tobacco mosaic virus particles, I can see the rod-shaped particles as indicated by blue...
22 September 2013 8,429 2 View
After regular PEG purification, can I use 25% chloroform (no phenol) to further purify the TMV virions from host proteins / free coat proteins? and what about getting rid of some remaining...
29 July 2013 4,790 5 View
I am working on replicating FHV in N. benthamiana leaves. The F1 RNA carries GFP. I had obtained a variation in results among different replicates: one batch having outstanding fluorescence, while...
27 June 2013 3,382 0 View
I started to work on a special gel electrophoresis with 0.5% agarose and TPE buffer (tris-Phosphate EDTA) to visualize some encapsidated TMV rods. So I made the 10X stock solution and diluted...
17 May 2013 6,704 3 View
I am working on tobacco mosaic virus in vitro and in vivo encapsidation. I mainly used PEG8000 to precipitate encapsidated virions. I am wondering if it's possible that PEG can also precipitate...
01 May 2013 8,286 2 View
We collected some wt TMV virions from tobacco plant leaves. We used 2X PEG precipitation and got a very large amount of virions on SDS-PAGE. When we proceeded to TEM to visualize the rod-shaped...
04 April 2013 3,578 8 View
Say that I have two sets of data, each with 3 biological repeats (unpaired). Dataset1: control:0.27/0.376/0.393 treatmentA: 6.285/7.574/18.171 unpaired two tailed t-test P = 0.051 > alpha...
01 January 1970 727 2 View
