I have Growth rate data for Listeria monocytogenes the same range of temperatures in two different media. I want to know if the growth rates is significantly different between the media - 1) at each temperature 2) over the entire range.
3) I also have the pH level for each experiment. If I want to see if pH had an effect on the growth rate.
Details: The growth rate is not linearly related to the temperature.
Could someone help me with the appropriate statistical test to be used for this?
Hi Dipon,
If you are measuring the growth factor from the same bacterium in two or more different media during a specified period of time, you could use repeated-measures ANOVA.
You need to first look at your data to make sure that they satisfy the assumptions for the ANOVA. Depending on your data, a non-parametric test may be better.
You could also use some regression to analyze the data. It all depends on the quetsion(s) you are trying to answer.
Best Regards
Yes, Dear
All factor (cultures media, pH and temperature ) affected on growth rate of bacteria
Hoda Sabati Thank you for your answer.
I have already fitted non-linear regression models (Baranyi and Roberts model) in my growth experiments to generate the growth rate.
My question now is if the growth rate under similar conditions of temperature and pH, different for the two media I have used.
Given this question, you would recommend a non-parametric test?
Dipon Sarkar Your welcome.
Based on the explanations you gave, I think non-parametric tests are more appropriate.
Good luck in your work.
Prepare a liquid samples of your media in an equal aliqouts.Then inoculate the media with your inocula at similar CFU per mL in similar volums.pH value and temperature should be similar and justified. Measure the OD at 800nm spectrophotometer and draw growth curves for each medium. Use the anova analysis to deduce similarities and disimilarities.
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