I am working with valproic acid and 5-Aza-2'-deoxycytidine. Perticularly, I wish to look for its impact on functional responses of cells. How I can see, whether my inhibitors are working or loosing stability?
Is it necessary to run the Rt PCR product on agarose gel after completion?
29 December 2020 4,823 3 View
I have downloaded the MSWEP sub-daily rainfall data (3-hourly data 0.1 degree resolution). I used MATLAB to convert it and obtained a table of dimensions 1800 x 3600 for a specific date. I am...
18 October 2020 4,205 4 View
I am trying to model a jet fan being installed in a room. I want to study the flow of the fan coming out of the outlet (basically the development of streamlines). The walls of the room are...
12 October 2020 4,970 3 View
I am new to abaqus and I am trying to optimize a shape of a segment of a model (As a part of my master thesis). Please refer to the example model I have attached. I need to optimize the segment...
23 August 2020 5,828 9 View
I have submitted one of my review article on COVID 19 and ophthalmology in April through online submission but journal haven't overlooked it inspite of several request. Even i have sent request...
06 August 2020 1,802 3 View
Hi I am trying to find some information as how to link up OpenFoam and Star CCM, however I am not able to find adequate information. Below is the details of the project. 1- Objective: to...
05 June 2020 8,484 3 View
What is your view to select an approach that also satisfy IoT constrains... 1) cryptographic approach or 2) certificate based approach or 3) blockchain based approach
10 May 2020 6,734 8 View
I am using python 3.6.8 on a Windows 10 machine. We can only move to subdirectories from the default installation (root) folder in jupyter. I wanted to start the notebook from a particular...
08 March 2020 9,259 4 View
HEMT FABRICATION.
24 February 2020 4,958 3 View
Hello, I am new to surrogate-based data analysis. In my model, Input space consists 6 variables and a single ouput is noted from each simulation. I have N such simulations. ( Sampling method is...
09 February 2020 7,511 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View