I am working with valproic acid and 5-Aza-2'-deoxycytidine. Perticularly, I wish to look for its impact on functional responses of cells. How I can see, whether my inhibitors are working or loosing stability?
I need to carry out antibacterial activity testing by disc diffusion or agar well diffusion followed by MIC by agar microdilution. The sample I have is a nanocomposit which doesn't dissolve in any...
16 February 2024 7,046 2 View
Hi all, I am having difficulty with relaxing my system using Q.E. I've tried several times but calculation just stops after some time duration. Last line of output file gives me "Davidson...
04 February 2024 5,788 0 View
One of the many assumptions in ANOVA is that our data follows a normal distribution. Usually in biology experiments are carried out in triplicates and the avalilable data is very small, not >...
28 January 2024 6,579 8 View
I need CIF file of Berlin Green.
21 January 2024 6,340 1 View
I have plant sample with mixed infection of CMV and potyvirus, is there any way I can separate CMV only?
10 January 2024 3,972 1 View
I'd prefer not buying any separate commercial media because of the cost. Please suggest what growth factors or vitamins can I add to DMEM KO other than glutamax, penstrep and bFGF for the MSCs to...
08 January 2024 6,443 3 View
I am analyzing RNA-Seq data, specifically focusing on the identification of variants within differentially regulated genes across treated and untreated samples. My primary analysis platform for...
01 January 2024 4,716 0 View
Civil remedies
01 January 2024 3,466 3 View
I am currently in need of an antibody targeting gene X, specifically focusing on a distinct splice variant where the major variance lies within the N-terminal region. Unfortunately, suppliers like...
26 December 2023 8,468 2 View
What tools or methodologies are available to comprehensively analyze RNA expression profiles across various human tissues, specifically to discern and compare transcript variants of a gene
19 December 2023 7,167 7 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View