I am having problems with high efficiency of my primers and one reason could be the formation of primer dimers.

I dont see any big peak on the melt curve, but I dont know if this increased peak is a primer dimer as well. Also, I dont see any smaller band in my gel.

What should I do?

I have used serial cDNA dilutions from 800ng RNA, 400, 200, 100 and 50, but with this I used up all 20uL of my RT sample. If I dilute 10x to get at least 200uL of cDNA, then I would have to decrease even more the concentration of my primers, right?

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