Simone Nascimento

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Questions related from Simone Nascimento

How to check for primer dimers?

I am having problems with high efficiency of my primers and one reason could be the formation of primer dimers. I dont see any big peak on the melt curve, but I dont know if this increased peak is...

20 September 2017 7,457 4 View

Checking RNA integrity for qPCR with electrophoresis but it does not show 28S/18S ~ 2. Should I extract again?

I have run RNA samples in 1% agarose gel but I cannot visually see a ratio 28S/18S ~ 2. What I might be doing wrong? Also, the samples marked with numbers, should I still consider them for qPCR?

13 September 2017 7,095 6 View