Hello Shobana, i do not see where you have the difficulty because the classical methods of protein separation and characterization have not changed, at least not to my knowledge. Protein characterization in recent times involves five protocols; protein quantification (involves Absorption at 280 nm, Lowry assay, Bradford assay and Mass Spectrometry - a technique by which you can determine the mass of a protein with remarkable precision. It can detect any important modification (post translational modification) or variation in a protein structure), Electrophoresis (migration of a protein in a gel as a function of size, shape and charge), dynamic light scattering (a technique used to determine the size distribution profile of small particles in solution) and circular dichroism (a variant of absorption spectroscopy which measures the difference in absorption of left and right polarized light (in the ultraviolet) by a medium. For protocols visit the links below:Article Isolation and characterization of soluble protein from the g...
Article Fractionation and characterization of soluble proteins from cider
https://www.uq.edu.au/pef/content/characterisation-techniques-0.
Hallo Shobana, as Maurice mentioned already, it might be a little difficult. I just wanted to add to in case your protein carries a chromogenic cofactor, it might be possible to characterize the specific protein which was over expressed. Best regards
Hello Shobana
Though different workable methods are there for your case, even if you are facing problem please be specific.
Thank you all.
I have to identify the proteins from the culture broth. Either by MALDI-TOF or by LC-MS
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