Why Cutting force coefficients or the edge coefficients will be negative in few cases of machining operation?

In mechanistic modeling of cutting forces in turning, identification of cutting force coefficients and edge coefficients through experiments are very much essential. these coefficients can be...

05 October 2019 3,864 3 View

How to solve scattering problem in sidewall of any pattern devices by using maxwell formulation without simulations through FDTD and COMSOL?

Actually i am looking some theoretical model who can solve the semiconductor scattering issues.

22 August 2018 3,062 2 View

Is it possible to get yield more than 100% in a chemical reaction....?

Is it possible to get yield of a chemical product more than 100%...? If so, how one can defend this situation? I am working on Condensation reactions using organic solvent free synthesis. For some...

22 June 2018 2,453 21 View

Can I transfect the only 147nt ssOdna 5'-3' (only sense strand)as repair template for Knockin experiment?

27 August 2017 3,856 2 View

Does anyone help me out to clone the Oligo sequence in Lenti CRISPRv2 backbone plasmid ?

18 May 2017 6,919 1 View

In ABAQUS: How to apply pressure loads at different locations?

In ABAQUS: How to apply pressure loads at different locations?

07 May 2017 1,676 4 View

Is there any way to in vitro methods for checking to anticancer properties of plant extracted substance without using animal cell line or MTT assay?

I am working on the phara quantification of plant substance, facing some experimental aspect , alike no availability of animal cell culture setup. So, anticancer assay aspect completely non in...

20 January 2017 8,296 5 View

What happens when the following events are noticed in Human Being?

What happens when: 1. HMGCS2 Expression is enhanced....? 2. PfA-M1 aminopeptidase is inhibited....? 3. Mcl-1 is antagonised (inhibited).....? (All these have been estimated using in-silico...

06 December 2016 487 1 View

How to use a mushroom as a drug developing tool ?

Mushroom drug discovery, human valuable substance etc.

24 July 2016 6,139 1 View

No title

30 March 2016 7,243 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View