How to calibrate a Microscan MS5608A (Elisa reader) instrument, which is not functional in last one year ?

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Where I can purchase a lower molecular weight cutoff membrane?

Please provide information on where I can purchase a 150 kDa, 500 kDa, 1000 KDa and 5000 KDa molecular weight cutoff membrane? for separation of ferulic acid (194 mwt) form mixtue of sample. where...

20 March 2024 2,818 3 View

Autodraw in OSLO software?

Hello everyone, I would like to ask you a very simple question about OSLO software for optical designing. Here I have attached an Autodraw window for an objective lens. Can anyone explain in the...

20 September 2023 6,348 2 View

Reseach Square is any legal agency of publication? Have any index and Impact Factor?

Why Research Square forces to researcher join the team? Its any useful? some papers online without acceptance in Research Square

05 May 2023 3,730 4 View

Can we calculate energy of any particular molecule?

If we take a protein or peptide, can we calculate energy without excited in higher stage?

31 January 2023 8,450 2 View

How to test laser intensity profile image is a Gaussian distribution ?

I am from Physics, background and doing some experiment using a laser source. Using a collimating lens, I have made the beam as a collimated beam. The intensity distribution of collimated beam is...

11 January 2023 7,347 11 View

How can we extract water soluble compounds during extraction highly polar compound like amino acid?

How can we extract water soluble compounds during liquid extraction but pH of Prduct highly basic up to 13 and product Highly soluble in water?

13 June 2021 5,736 3 View

Many people recovering from COVID-19 have of late been afflicted by black fungus or mucormycosis disease. What can be the exact reason?

Recently in India many people recovering from COVID-19 have of late been afflicted by black fungus or mucormycosis disease. What can be reason? how to diagnosis in early stage?

17 May 2021 6,958 26 View

What is main difference between COVID-19 effected patient fever body temperature and typhoid fever body temperature?

We are writing report on COVID-19 fist, second and third wave in different country of the world.

21 April 2021 4,665 2 View

How to extract the on current and off current of TFET form the output results Vds vs Ids and Vgs vs Ids in silvaco Atlas?

i am trying to know the On current and off current of TFET form its characteristics in silvaco Atlas?

19 April 2020 4,453 1 View

How to add a user defined material with high dielectric value in silvaco atlas ?

procedure to add different high dielectric constant material in the silvaco atlas?

16 January 2020 3,629 5 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

Has anyone used the new E1 Clip tip digital pipettes?

We just purchased a multichannel and a single channel E1 Clip tip pipettes. In our first attempt to use them for an experiment they are not dispensing accurately? The rep told me that...

01 July 2024 1,983 0 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Different interpolated concentrations?

I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...

15 June 2024 5,109 1 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View

How to convert mIU/mL of beta galactosidase enzyme to pg/ml? I have used ELISA to determine beta gal concentration in mIU/ mL in human serum samples?

We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?

05 June 2024 2,173 5 View

What is Area Under the Curve for detecting SARS-COV-2 neutralizing antibodies?

I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...

05 June 2024 8,191 0 View

Mike Zhang

Contacting the manufacturer's customer support might be helpful.

Nagendra Kumar Chandrawanshi

Thank You, Miike, I have contact manufacturer, but I to want know, from the user for the same instrument.

Khaled Elnagar

If it is not functional in last year...it shall be checked by manufacturer... most of these microscan has auto-adjustment or according to their operating manuals...