Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...

01 February 2021 5,809 3 View

Is it reliable to use toluene as a solvent in measuring density by liquid displacement method?

Hi all, I am working on pollen and want to examine the true density. No doubt, toluene does not swell the particles but it may dissolve the lipid materials and other organic molecules besides the...

07 January 2021 2,471 2 View

Are hydrogels really important for food processing sector ?

Dear all, I am just reading about a new trend in food sector i.e. developing hydrogels. Literature showed uses of hydrogels in food packaging and bioactive molecule delivery or their carrier in...

27 December 2020 4,392 6 View

Fluorescence spectra problem during protein ligand interaction??

I checked protein ligand interaction through Fluroscence, I keep my protein concentration constant with varying concentration of ligand at the small concentration 5uM the FLuroscence is peak is...

21 December 2020 8,609 4 View

How to identify the compounds present in the methanolic extract of Artemisia leaves using LC-MS?

I have done LC-MS of the methanolic extract of Artemisia leaves. I have got the chromatogram, but due to the unavailability of the mass library I am not able to interpret the results. How can I...

14 December 2020 2,446 4 View

Can we solate viable cells from frozen xenograft tissues?

I am planning to isolate cells from frozen xenograft tissues (stored in liquid nitrogen). The tissues are stored in 10% DMSO, 30%FBS and 60% RPMI with Penn/Strep. These tissues were frozen with a...

14 December 2020 3,910 1 View

Which are the post harvesting processes of turmeric which results in maximum loss of curcumin content of Turmeric?

I am talking about the GI tagged Kandhamal Haladi (Turmeric), which is famous for its curcumin content.There are few blocks in Kandhamal district such as Balliguda where curcumin content is very...

18 November 2020 2,304 3 View

Why not there is common reference citation format for all journals?

Why not there is common reference citation format for all journals?

03 October 2020 8,137 3 View

Hello Everyone, Can anyone help me in the transfection procedure of plasmid into chicken spermatozoa by electroporation?

Hello everyone, I am doing an experiment in which, the plan of work is to electroporate a clone (plasmid form) into chicken spermatozoa, which will be used further for artificial insemination....

07 September 2020 7,855 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View