Hi guys,
I'm working on a mouse model (using C57BL/6J) that needs high-fat diet (HFD). Recently I plan to perform ipGTT, just testing blood glucose. My colleague thought 1g of glucose/kg body mass was fine.
But some research suggested a higher amount of glucose for those mice fed with HFD.
One study recommended 1.5-2g/kg. (doi:10.2147/DMSO.S234665)
Another study suggested 2g/kg, but orally. (doi:10.1152/ajpendo.90617.2008) Can anyone explain the reason why we should choose a higher dose of glucose? Or maybe 1g/kg is also appropriate?
Many thanks. .
Please check journal. This is very important factor to decide our work. Most of the time low impact factor journal published without giving appropriate information.
I read some papers and there are different methods. Some choose 1.5g/kg and others choose 2g/kg.
But my colleague adopted 2g/kg before and she found the blood glucose was so high that she couldn't measure the glucose levels from the meter. Maybe I choose 1.5g/kg to check it. Thank u so much. Mohammed Kaleem
Kindly use 2g/kg is appropriate. I have used in my obesity Project last year.
Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral
Glucose Tolerance Test (OGTT) and Insulin Tolerance. Read this article
Did you get data from the glucose meter when you used 2g/kg before? Actually, my colleague also did it, but she failed because it's too high and we didn't know why. Do you remember the brand of meter you used? Many thanks. Mohammed Kaleem
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908452/: DOI:10.3791/56672.
Preparations on the day of the experiment (but prior to the experiment)
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