16 Questions 20 Answers 0 Followers
Questions related from Tingting Wang
Hi, I found more monocytes in the heart in treated group accoring to the FACS data. And I did qpcr to validate this result. First I checked CCL2 (or MCP-1) and other cytokines/chemokines because...
21 October 2024 9,938 5 View
Hi, I fed mice (6N) with HFD (research diets, D12492) and L-NAME (0.5g/l) to get HFpEF mouse model, but those mice didn't gain too much weight. I know some people also used HFD to establish...
05 September 2024 4,838 5 View
Hi, I plan to sacrifice 5 mice in one day, and I want to take some organs. I’ll also collect blood and bone marrow for flow cytometry. But I don’t know how to arrange that properly because all...
13 August 2024 6,661 5 View
Hi, I'm working on heart flow cytometry, and I use anti-mouse CCR2-BV785 (biolegend, cat150621) for staining, 20 minutes, 4 degree. But I couldn't see any CCR2+ cells. So I'm not sure if it's...
04 July 2024 4,087 4 View
Hi, I'm working on the immune cells in the heart and have a problem with CCR2 marker. I did some research and found there's CCR2+ monocytes in heart failure mouse model. When I analysed CCR2...
09 June 2024 7,645 5 View
Hi guys, I'm working on the tumor mouse model. In general, I prepared tumor cells and injected cells into the flank of mice subcutaneously. After around 3 weeks, I found, in the same group,...
01 March 2024 9,080 3 View
Hi, I'm new to flow cytometry. Basically, I measured immune cells in the heart using the BD device. And I found sometimes the range of threshold rate was not similar among samples. For example, I...
22 November 2023 990 1 View
Hi, I'm working on the immunity of tumor. I injected tumor cells into the mice subcutaneously. After harvesting the tumor, I used percoll and isolated immune cells. Then I added trypan blue and...
21 November 2023 7,716 4 View
Hi there, I saw C57BL/6 was the most common mouse strain to be used as heart failure mouse model. I was just told that Balb/c was not suitable for heart disease, and acutally I don't know...
22 June 2023 412 3 View
Dear all, I saw the 3LL could be identical to LLC1 on the website (https://www.cellosaurus.org/CVCL_4358). But I didn't find any information about those two cell lines in published papers. Can...
08 May 2023 9,106 0 View
Hi, I'm working on lung cancer cells, LLC1 (CRL-1642, ATCC) but I'm not familiar with it. which media and what kind of FBS are good for LLC1? or are there anything important I need to know? Many...
05 May 2023 1,185 3 View
Hi there, I used L-NAME to treat cancer cells. Then I did LDH and MTT, according to these results, MTT showed the cell viability was above 100% compared with control, and LDH showed cytotoxicity...
31 March 2023 7,565 3 View
Hi guys, Does anyone do echo for the HFpEF mouse model? I'm doing the echo for the HFpEF mice models which are fed with HFD and L-NAME. Those mice are under anesthesia for echo. And it's...
19 November 2022 7,959 0 View
Hi guys, I'm working on mouse tumor models and I planned to inject tumor cells into C57BL/6 mice. Recently I found that tumor cells are derived from BALB/c mice. I also read some papers and...
15 November 2022 7,376 0 View
Hi guys, I'm working on a mouse model (using C57BL/6J) that needs high-fat diet (HFD). Recently I plan to perform ipGTT, just testing blood glucose. My colleague thought 1g of glucose/kg body mass...
09 November 2022 3,693 7 View
When we kill mice and take their tissues (live, kidney, fat, etc), not cells, we put them in cryogenic tubes respectively and then put tubes in the liquid phase of liquid nitrogen for short-term...
31 July 2022 6,043 4 View
