Hello lovely people,
It is my first question here, so I hope you can help me..
For an experiment of my thesis I provided pure cultures with different concentrations of lactate an monitored them over time using HPLC. I now have the Lactate concentration within the medium for the pure cultures, as well as for abiotic control bottles. Both show a decline in lactate (stronger in inoculated bottles), but how can I know calculate the lactate consumption rate of the bacteria? What is the best way to display bacterial lactate consumption? So far I never did such plots and am very confused what to do next.
Please look at the following below links which may help you in your analysis:
https://journals.plos.org/plosgenetics/article/file?type=supplementary&id=info:doi/10.1371/journal.pgen.1006372.s013
http://cmbe.engr.uga.edu/bche8210/SectionB.pdf
https://www.sciencedirect.com/topics/engineering/substrate-consumption
Thanks
Just measure it spectrophotometrically using a kit or automated blood lactate analysers or strips .....Many methods are available, you can also measure a decrease in lactate concentration via lactate metabolizing enzyme.
Article Blood Lactate Measurements and Analysis during Exercise: A G...
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