Sample dry weight is 15.8 mg; Volume of extraction buffer is 1 mL.
After Bradford Assay, sample concentration is 3.65 mg/mL. How to find protein percentage from this data?
Determine the total amount of protein extracted by multiplying the concentration by the volume of extraction buffer used, which gives you 3.65 mg of total protein. Then, divide this by the dry weight of your sample and multiply by 100 to get the percentage. So the protein content of your sample is (3.65 / 15.8) × 100 = 23.1% of the dry weight.
This is a very basic homework question. Go check your notes on how to do the math
Kiran Reddy ,
The sample dry weight is 15.8 mg; the extraction buffer volume is 1 ml.
After Bradford determination, the sample concentration is 3.65 mg/ml.
The sample concentration is 3.65 mg/ml and the extraction buffer volume is 1 ml. Multiplying the concentration by the extraction buffer volume determines the total mass of protein extracted, which is 3.65 mg total protein.
Then, the sample dry weight is 15.8 mg, and this value is divided by the sample dry weight to obtain the sample protein percentage content, which is (3.65 / 15.8) × 100 = 23.1%.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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