Kiran Reddy

4 Questions 1 Answers 0 Followers

Questions related from Kiran Reddy

How to calculate protein percentage after Bradford assay?

Sample dry weight is 15.8 mg; Volume of extraction buffer is 1 mL. After Bradford Assay, sample concentration is 3.65 mg/mL. How to find protein percentage from this data?

29 May 2025 1,408 3 View

How to calculate total protein content (mg/gDWt) after Bradford Assay?

Tissue used (dry legume seed powder): 15.7mg, 15.4mg, 15.9mg BSA concentration for standards (0.5, 1, 1.5, 2 μg/mL) 500μL 0.1M NaOH used as extraction buffer 20μL of protein extract and 200μL...

21 March 2025 7,100 1 View

Can I store lyophilized cowpea powder in room temperature or it should be under -20C for amino acid quantification?

I lyophilized cowpeas and powdered then using mortar and pestle. How to store the powder until further protein and amino acid quantification?

13 January 2025 2,819 2 View

Does 260/280 ratio between 1.71 - 1.85 acceptable for further DNA downstream analysis?

I am using Qiagen Plant DNA kit for extracting Tepary bean (Phaseolus acutifolius) DNA. For 260/280, I am getting a ratio between 1.71 - 1.84 and concentration between 50 - 200ng/µL

29 December 2024 7,079 1 View