How to calculate molarity of a homotetrameric protein from a given concentration obtained via nanodrop?

26 January 2022 1 10K Report

I'm purifying a protein which is homotetrameric in nature and each subunit is catalytically active. However, I am a bit uncertain about the molecular weight which I should consider upon calculating the molarity of the given protein because what I believe is that each monomer gets formed independently and then form dimers to attain dimeric or tetrameric configuration.

I do have the concentration which I have obtained via nano-drop. However to obtain the molarity I am not sure to use molecular weight of a monomer (which is catalytically active) or a tetramer?

Abdelkader BOUAZIZ

Dear Muhammad Yousuf, please have a look at the following documents. My Regards

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