this will give you a brief example: http://lukemiller.org/index.php/2010/02/calculating-lt50-median-lethal-temperature-aka-ld50-quickly-in-r/

An internet search will provide you with many options and specialised R packages for survival/toxicity analysis.

Dear Sukhjinder Mann ,

Basically its a graphical method for calculating the LD50. In this we plot Log dose or Log concetration on the X-axis Vs Probits of mortality on the Y-axis, for varied range of doses. Finally we mark the 50% unit on the Y-axis and extrapolate this to the log dose or log concentration marked on the x-axis.

Here first, we need calculate % mortality, then convert the % mortality to probits. The probits should be plotted on the Y-axis as stated before.

Please refer the link for details.

http://userwww.sfsu.edu/efc/classes/biol710/probit/ProbitAnalysis.pdf

https://ncss-wpengine.netdna-ssl.com/wp-content/themes/ncss/pdf/Procedures/PASS/Probit_Analysis.pdf

Regards

Vishwanath

I use probit analysis on SPSS to calculate LD50, see details in this PDF:

See the details in the attached file.

Look, please, following papers.

there are many software available. you can also calculate from excel. see the lectures in youtube

i also need software for the probet calculation

To find LD50 from probit calculation, there are probit softwares and the book of Finney D. J. 1971(Probit Analysis, third edition, Cambridge University Press, London).

I agree with Munira and send you my paprer about calculation LD50 and other LD values using methods of Finney and Miller-Taitner, using MATLAB. Send programs for calculation LD50 and other LD values in MATLAB to.

Use the R program

Halla Abdul-Hadi Chabuk

Help.

Calculating LC50 using genstat

I suggest you use genstat or on the alternative you consult Finney,D.J. 1971

you can use spss also.

I use Ldp line. Very easy to calculate it.

http://www.ehabsoft.com/ldpline/

You can use any specific for soft-ware for Probit Analysis. General statistical soft-ware like SAS, SPSS will also help.

Use Software-EPA Probit Analysis Program (version 1.5).

Use the PoloPlus of the Leora Software or SAS program.

SAS software gives a sigmoid LDP line. In my experience, I prefer MINITAB software for calculating the LD50.

The xlstat is useful to

Use xlstat or spss

Use SPSS which is having elaborative output, and similarly CCSHAU's OPSTAT also upgraded their online software for analyzing probit (free to all users) and no download required.

Thank you

Please follow Dr. M.R Sreenivasan's google site. You will get an excel worksheet for estimation of LD50 and LC50.

You can download this excel