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Questions related from Vishwanath shastry G.L.
How can I prepare cell lysate from rat brain homogenate for estimating the levels of nuclear factor-kappa B (NF-kB) using ELISA kit? Looking for freezer thaw cycles method in detail.
29 January 2019 6,176 4 View
The NIOSH draft guideline highlights the importance of OEB and its applications with examples. However, still there is lack of clarify on how to obtain/derive OEB when we have OEL and...
26 November 2018 1,173 0 View
In deriving the Acceptable Daily Exposure (ADE) and Permitted Daily Exposure (PDE) of a chemical entity, the EMA and ISPE-MaPP guidelines mention 50 Kg body weight, any scientific rationale...
16 October 2018 7,762 0 View
What are the best statistical tools to analyse the reliability and validity of a diagnostic instrument?. Which gives the data in the ratio scale and the instrument is used for measuring the as...
22 September 2018 242 4 View
Hi Colleagues, Can anyone suggest me any free statistical software for performing Quality Control Analysis involving Six-sigma principles. Regards Vishwanath
01 January 1970 8,371 9 View
Dear Colleagues, What are the general considerations for PDE calculations of Ophthalmic preparations, and are there any specific guidelines or literature on these, please...
01 January 1970 5,261 1 View
Dear Colleagues, Is it acceptable to define the OEB category of a chemical using its OEL value? As per the NIOSH guideline, there are 5 categories (A-E) given and under each category, different...
01 January 1970 1,654 4 View
Dear Colleagues, We all know that, Pharmacokinetic and Toxicokinetic properties of a chemical entity will have pivotal role in deriving the ADE/PDE/OEL and other health based exposure limits....
01 January 1970 797 6 View
I am currently working on ischemia- reperfusion-induced brain injury in Wistar rats, and as of now we are using Ketamin-Xylazine or low dose of Urethane for inducing anesthesia. I have over heard...
01 January 1970 652 6 View
01 January 1970 8,465 4 View