Vishwanath shastry G.L.

10 Questions 47 Answers 0 Followers

Questions related from Vishwanath shastry G.L.

How can I prepare cell lysate by freezer thaw cycles method, from brain homogenate for estimation of nuclear factor-kappa B (NF-kB) using ELISA kit?

How can I prepare cell lysate from rat brain homogenate for estimating the levels of nuclear factor-kappa B (NF-kB) using ELISA kit? Looking for freezer thaw cycles method in detail.

29 January 2019 6,186 4 View

How to derive Occupational Exposure Band (OEB), when we have OEL?

The NIOSH draft guideline highlights the importance of OEB and its applications with examples. However, still there is lack of clarify on how to obtain/derive OEB when we have OEL and...

26 November 2018 1,178 0 View

Body weight considerations in ADE and PDE calculations?

In deriving the Acceptable Daily Exposure (ADE) and Permitted Daily Exposure (PDE) of a chemical entity, the EMA and ISPE-MaPP guidelines mention 50 Kg body weight, any scientific rationale...

16 October 2018 7,767 0 View

What are the best statistical tools to analyse the reliability and validity of a diagnostic instrument?

What are the best statistical tools to analyse the reliability and validity of a diagnostic instrument?. Which gives the data in the ratio scale and the instrument is used for measuring the as...

22 September 2018 247 4 View

Best statistical Package for performing Quality Control analysis

Hi Colleagues, Can anyone suggest me any free statistical software for performing Quality Control Analysis involving Six-sigma principles. Regards Vishwanath

01 January 1970 8,377 9 View

PDE calculations for Ophthalmic preparations

Dear Colleagues, What are the general considerations for PDE calculations of Ophthalmic preparations, and are there any specific guidelines or literature on these, please...

01 January 1970 5,265 1 View

How to define OEB category of chemical using OEL?

Dear Colleagues, Is it acceptable to define the OEB category of a chemical using its OEL value? As per the NIOSH guideline, there are 5 categories (A-E) given and under each category, different...

01 January 1970 1,659 4 View

Toxicokinetic (TK) factor consideration in calculating Occupational Exposure Limits (OEL) of a substance.

Dear Colleagues, We all know that, Pharmacokinetic and Toxicokinetic properties of a chemical entity will have pivotal role in deriving the ADE/PDE/OEL and other health based exposure limits....

01 January 1970 802 6 View

What is the Best Anesthetic agent for performing surgeries related to ischemia-reperfusion and related brain injuries in Wistar rats?

I am currently working on ischemia- reperfusion-induced brain injury in Wistar rats, and as of now we are using Ketamin-Xylazine or low dose of Urethane for inducing anesthesia. I have over heard...

01 January 1970 657 6 View

Toxicokinetic (TK) factor consideration in calculating Occupational Exposure Limits (OEL) of a substance.

Dear Colleagues, We all know that, Pharmacokinetic and Toxicokinetic properties of a chemical entity will have pivotal role in deriving the ADE/PDE/OEL and other health based exposure limits....

01 January 1970 8,470 4 View