How can I validate the UV spectroscopy?

can I compare UV spectroscopy reading with nanodrop reading to validate the UV spectroscopy? or is there a better way to know if the UV works well or not? Note: we use it in DNA, RNA &...

12 December 2020 4,869 2 View

What's the right way to Tween 20 storage?

02 December 2020 9,955 2 View

What's makes RNA (and not DNA) bind to the silica spin column in commercial kits?

In silica spin column-based kits, if the spin column material is the same and the binding to the column occurs by using ethanol and Guanidine salts. Then, what's the difference between the RNA...

20 September 2020 4,798 1 View

Is it possible to apply statistics on synthesis and anticancer activity?

I m looking for paper having knowledge of statistics (spss or matlab) applied on synthesis and anticancer activity

20 March 2020 5,517 3 View

Why Non specific bands of PCR reaction appear while using specific primers for tobamoviruses?

i have done RNA extraction and cDNA for tobamovirus then i made a PCR reaction using primer pair Tob uni1/Tob uni2 according to the protocol in Letschert et al., 2002 ( annealing temp. 60C for 45s...

09 December 2019 7,276 3 View

I would like to know about how can crystallization of metal complexes take place?

When i prepare the complexes it forms in amorphous state i want to make crystals of complex.my complexes soluble DMSO but when i dissolved in it and pour insoluble solvent it was oxidized.

15 February 2019 7,053 2 View

Help Please!! how to solve the error "too many attempts made for this increment" in abaqus?

i am applying a load of 50KN gradually on a reinforced concrete beam, i'm unloading this load using an amplitude to get the loading and unloading versus deflection graph.i have used concrete...

09 June 2017 3,129 14 View

Hello How to get a producer to dissolve cellulose fiber by carbon disulfide ?

11 April 2017 9,899 2 View

How to get the frequencies of simply supported RC beam when changing load in ABAQUS?

i have modeled the RC beam with simply supported BC and used concrete damaged plasticity model to account for the nonlinearities in concrete, i have got the frequency value for the case of beam...

01 January 2017 737 6 View

What kind of experiment help in understanding the metabolic pathway in plants?

In my proteomic results, there is change in glycolysis and TCA and i want to know that which pathway is involved in changing these major metabolic processes. For that i want to do some more...

18 September 2016 396 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

Any reference paper with detailing on how to hybradize Q-Learning algorithm with Genetic Algorithm, especially for a variable optimization problem?

I am planning to hybradize Q-Learning algorthm with Genetic algorithm at the population initialization stage, crossover stage and mutation stage. The problem under consideration is a...

07 February 2021 3,123 8 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

How can I get the chromosomal location of a list of genes?

I'm looking for a bioinformatics website to get the chromosomal location of a radish gene id list, but in one shot instead of doing it gene by gene. Is there a way to do this? Thanks

17 January 2021 8,810 3 View

Are you equipped to count small plasmid chromosomes?

I wish to perform an experiment which will provide support for a novel DNA structure. Most of the experiment is conceptually simple, easy to perform, and inexpensive. But the key to doing it is...

29 November 2020 5,258 9 View

What is this citation style called?

Hello everyone, I am preparing a manuscript for a journal which indicates in the authors guidelines that the citation and referencing style should look like the following: Citation in the text...

22 September 2020 6,897 6 View

What can I do if I get unexpected plasmid after sequencing of bacterial DNA? how can I characterise the unknown plasmid?

After sequencing a bacteria Streptococcus iniae I have got a chromosomal DNA and a extra plasmid DNA. I have not found any evidence of Streptococcus iniae having plasmid!! is it possible to...

02 July 2020 6,319 8 View

How genetic algorithm can be used to find optimal weights and biases of neural network?

Assume a 3-2-1 (3 neurons in the input layer, 2 neurons in the hidden layer and 1 neuron in the output layer) neural network. How genetic algorithm can be used to find optimal weights and biases...

24 May 2020 1,778 2 View

How to extract info from a merged VCF file of annotated WGS analyses in galaxy?

I have a joint flagged tab VCF in which are reported the annotations and variants of 69 samples. How can i extract information for a single sample or chromosome in Galaxy?

21 April 2020 3,836 1 View

What would happen in the chromosome if you had a single crossover between homologous parts of linearised plasmid into chromosome?

The plasmid being used is pBS1 and it has 2 segments of DNA on it which are derived from the B.subtilis amylase gene. There is a chloramphenicol resistance marker inserted between the amyfront and...

18 March 2020 8,875 2 View