Hello Sarnam Singh
Nucleolar organizer regions (NORs) are defined as nucleolar components containing a set of argyrophilic proteins in two nucleolar regions namely, the fibrillar centers and the dense fibrillar component. These two regions are directly involved in the transcription of rRNA. NORs are present in the nucleoli of cells in interphase.
NORs contain acidic, non-histone proteins which have increased affinity for silver ions. Therefore, staining with silver acetate enables easy visualization of NORs. After silver-staining, the NORs can be easily identified as dark granules exclusively localized throughout the nucleolar area and are called AgNORs. The intensity of staining is dependent on the transcriptional activity of the cells.
FYI: NOR banding stains only those regions that were active as nucleolus organizers in the preceding interphase as well as useful for visualization and study of satellite associations.
You may want to refer to the article attached below for more information.
Article [Silver staining of nucleolus organizer regions (NORs). Appl...
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