I am doing biochemical assays for insecticide resistance in Aedes aegypti populations, micoplate assays, but I am not clear how to calculate the enzyme activity for each assay. I was wondering if I could get in touch with someone who could guide me, since is the first time I do it. Thank you very much.
First it is important to define what technique you are using for the biochemical tests, and what families you are quantifying. You must do calibration curves, correct in relation to the total volume of the homogenized, consider the substrate used, as well as coefficient of extición etc. according to the case. See (Valles et al., 2006).
Yamili Contreras-Perera Thank you Yamili for your answer. I am doing microplate assays, as described by Brogdon, and I have done all the calibration curves. I am just not sure about doing the data analysis, the calculations. Since there is a lot of detailed information about the procedure of doing the assays, but not how to calculate after you get the absorbance. I really need someone to confirme the logic and process I am using to calculate my activites. If you think you could help me, I would really appreciate it!.
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