How to build 4T1 tumor in mouse?

10 October 2017 1 7K Report

There is orthotopic injection of 4T1 into mammary fat pad, there is also subcutaneous injection to the mammary region. I also see people use 4T1-luc for i.v tail injection(I don't have 4T1-luc cells so may not consider this one).

I have 4T1 WT and a gene knock down 4T1 cell line. Just want to see any phenotype after the gene knock down. Which is the best way to build 4T1 tumor in mouse?

Mengyu Wang

I would like to recommend you to consider "Fat Pad" model when you do your test. The reason is that FP model can have stable tumor growth than other model.

Good luck,

Mengyu

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