Statistical test: 1 nominal independent variable and 1 dichotomous dependent variable?

I have a dataset with one nominal independent variable with 10 different levels and one dichotomous dependent variable. What would be the appropriate statistical test to compare the different...

09 December 2020 8,834 29 View

Do you run Gen Set Analysis (GSEA) or pathway analysis (EnrichR, ROntoTools ++) on gene expression data??

Hi, im newbee for analysing gen expression data set and hope someone have som advise. Im working on mice liver gen expression (mRNA) data sets generated with EdgeR - now I want to do some gen...

03 September 2020 1,271 3 View

Why does spectral binning in CHENOMX seem to ignore the spectra baseline?

I am new to NMR analysis. What I wish to do is an untargeted approach (i.e. no compound identification), using standardized spectral binning. This should be possible and in theory pretty...

19 November 2019 9,402 3 View

How to distinguish somatic cells from spermatozoa when counting using flowcytomatry?

Hi, Im in urgent need of some guidance regaring flowcytomatry. Im using this method to quantity the amount of spermatozoa and somatic cells in my samples. I have stain with Hoechst 33258, and...

19 August 2018 9,915 3 View

RNA isolation from sperm within frozen cauda and vas?

Hi, do anyone have experience with isolation of mice RNA from sperm in snap-frozen cauda and vas? The plan is to thaw the cauda, squeeze the sperm out, remove somatic cells before sperm lysis...

03 April 2018 6,888 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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02 March 2021 3,060 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View