Dear Mathilde,

any binning I know of simply sums up all datapoints in the bin and if this sum is negative you'll get a negative value. What is of key importance therefore is to have a flat baseline which normally is achieved by proper phasing (not done in the data you show!), baseline correction (e.g. stripwise polynom) - unfortunately reliable algorithms that can do this with high precision and in automation for a set of spectra are not available (to my knowledge). But most important is a suited pulsesequence with adequate water/solventsupression (mostly noesypr1d or something linke this)....I hope this helps.

Regards

Alfred

Dear Alfred,

Thank you for your answer.

But, say I didn't even needed the quantification a simple presence/absence of the peaks per bin would suffice. In that case, I am thinking it must be technically possible to draw a line at a given intensity level ("y-axis") i.e. forcing not so much a baseline but a 'zero'-line and ignoring everything below that line in a binning approach.

Or am I missing something that will make such an approach impossible?

Best,

Mathilde

..hmm..conceptionally you are right, but what would happen to signals so small that they remain completely below the baseline (see e.g. duplett of duplett (dd) and singulett (s) left and right of your signal of interest - if you were interested in those)...I'd regard such a approach "dangerous" - and would recommend properly processed spectra!

Alfred