Hildegunn Dahl

6 Questions 6 Answers 0 Followers

Questions related from Hildegunn Dahl

Double strand breaks (DSB) and ATAC-Seq?

Is it possible to predict double strand breaks (DSB) (or repair??) using only ATAC data?

08 October 2021 4,234 3 View

TBARS in liver tissue?

Hallo experts, How can I best measure damage after oxidative stress in liver tissue harvested 24h after exposure stop? Im thinking about TBARS Elisa kit, but maybe anyone have advice...

16 November 2020 6,411 3 View

Do you run Gen Set Analysis (GSEA) or pathway analysis (EnrichR, ROntoTools ++) on gene expression data??

Hi, im newbee for analysing gen expression data set and hope someone have som advise. Im working on mice liver gen expression (mRNA) data sets generated with EdgeR - now I want to do some gen...

04 September 2020 1,388 3 View

How to distinguish somatic cells from spermatozoa when counting using flowcytomatry?

Hi, Im in urgent need of some guidance regaring flowcytomatry. Im using this method to quantity the amount of spermatozoa and somatic cells in my samples. I have stain with Hoechst 33258, and...

20 August 2018 10,005 3 View

How to best seperate somatic cells from mice sperm cells?

I`m squeezing mice sperm cells from cauda and vas that has been frozen - from this I will isolate sperm miRNA. My problem: How to best remove the somatic cells from the "squeeze"? Im using...

05 April 2018 4,548 3 View

RNA isolation from sperm within frozen cauda and vas?

Hi, do anyone have experience with isolation of mice RNA from sperm in snap-frozen cauda and vas? The plan is to thaw the cauda, squeeze the sperm out, remove somatic cells before sperm lysis...

04 April 2018 6,987 8 View