Currently we have a two-chamber organ-on-a-chip setup of three cell types (immune cells+cell type A in one chamber, cell type B in another chamber) where we want to measure immune cell migration from one chamber to another. Currently we are measuring immune cell migration in terms of absolute number of cells migrating but in such cases, we have to image the entire setup under a microscope. Is there a scoring system for immune cell migration?
I think the way you are doing it is probably the best way (if you use an insall chamber for the microscopy and imaging).
The alternative would be to count the cells after the experiment
It's relevant to have a better understanding about the context and your hypothesis to be tested here. Because you can measure migration by different ways. For example, with Boyden chamber, transwell chamber or adoptive cell transfer in a animal model. If you can provide here more info regarding the question to be answered I can be more precise. If you prefer contacting by private message you are welcomed.