Michael J V White

3 Questions 18 Answers 0 Followers

Questions related from Michael J V White

How could i measure the relative proportions of bound amino acids in neutrophil granules?

Hi all, How could i measure the relative proportions of bound amino acids in neutrophil granules? I'm looking for the proportion of all 20 of amino acids incorporated into proteins within a PMN...

10 June 2021 3,044 0 View

Does cy7 survive the process of paraffin mounting?

Hi, I'm looking to mount lungs and kidneys that I've targeted a cy7 fluorescent protein to. My question is, will cy7 survive the process of paraffin mounting, and remain fluorescent? While the...

10 September 2020 1,966 1 View

Can anyone explain why an unusually high percentage of monocytes are dying in my spleen-derived monocyte preps?

Hi all, I’m trying to purify monocytes from mouse spleens. However, in these preps an unusually high percentage of monocytes are dying, and thus I’m not getting a good yield for the...

10 April 2019 1,514 4 View