Pros and cons of keto diet?

health professionals have different opinions on keto diet. Some highly encourage it others suggest to avoid. Please provide your opinion or research with reference thank you

04 January 2021 8,056 10 View

Inverted peaks in the Imagelab lane profile?

Hello everyone, I have TIFF images of western blots that were acquired using Syngene chemidoc machine. I have converted TIFF files into 8-bit images using ImageJ (as there was a error opening the...

14 September 2020 4,881 5 View

The United States, China, and Thucydides Trap: Do you think there will be a war between China and the United States?

I think both countries are going to adopt solution, as the main reason for adopting the solution is obvious: nuclear deterrence - every nuclear power has the power to annihilate humanity. So it's...

07 September 2020 7,835 19 View

How can I extract shoreline/coastline from landsat data?

Please describe NDWI, MNDWI, AWEI, Geometric rectification

15 June 2020 7,747 7 View

Is it possible to check the "Endogeneity" Problem by running a correlation among all explanatory variables and error term?

As we all know, if all explanatory variables are correlated with the error term, we called it the endogeneity problem and its against the Exogeneity assumption of OLS regression. I just want to...

14 December 2019 9,785 7 View

Problems with 3T3-L1 Adipocytes?

Hi there, I am new working with 3T3-L1 Adipocytes I am facing some issues with these cells. Can anyone suggest me how to overcome this? I purchased 3T3-L1 cells from ATCC and cultured these cells...

04 December 2018 475 10 View

Why Hall Coefficient vary from positive to negative randomly for the same sample at same condition ?

We use a HMS 3000 Version 3.51.5 machine for Hall Effect measurement in our lab. Why does it show positive and negative Hall Coefficient randomly in different for the same sample at the same...

03 October 2017 1,455 7 View

How can I do a chi squared in SAS that accounts for unequal sample sizes?

21 March 2016 1,714 3 View

Why did Microsatellite amplification succeed in some samples of the same species and failed in others?

Hello every body I am working in a project involving screening of spiny lobster using 100 microsatellite groped into multiplexes.. During my research, I faced some markers that succeed in some...

22 April 2015 3,090 12 View

What is the best way to go around in solving a structural problem using the explicit method on ANSYS?

I am using ANSYS APDL to conduct a structural analysis on a structure under an axial compressive load to be able to determine its ultimate collapse strength and failure mode and I want to use an...

24 June 2014 6,711 6 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View