Pros and cons of keto diet?

health professionals have different opinions on keto diet. Some highly encourage it others suggest to avoid. Please provide your opinion or research with reference thank you

04 January 2021 8,056 10 View

Inverted peaks in the Imagelab lane profile?

Hello everyone, I have TIFF images of western blots that were acquired using Syngene chemidoc machine. I have converted TIFF files into 8-bit images using ImageJ (as there was a error opening the...

14 September 2020 4,881 5 View

How can I extract shoreline/coastline from landsat data?

Please describe NDWI, MNDWI, AWEI, Geometric rectification

15 June 2020 7,747 7 View

Problems with 3T3-L1 Adipocytes?

Hi there, I am new working with 3T3-L1 Adipocytes I am facing some issues with these cells. Can anyone suggest me how to overcome this? I purchased 3T3-L1 cells from ATCC and cultured these cells...

04 December 2018 475 10 View

Why Hall Coefficient vary from positive to negative randomly for the same sample at same condition ?

We use a HMS 3000 Version 3.51.5 machine for Hall Effect measurement in our lab. Why does it show positive and negative Hall Coefficient randomly in different for the same sample at the same...

03 October 2017 1,455 7 View

How two non polar groups interact?

21 October 2016 8,783 3 View

How to arrest Metaphase cells in culture?

I wonder if any one can help me with protocol for Chromosome preparation to analyse through Flowcytometry for plant cells

29 May 2016 4,923 3 View

How can I do a chi squared in SAS that accounts for unequal sample sizes?

21 March 2016 1,714 3 View

Why did Microsatellite amplification succeed in some samples of the same species and failed in others?

Hello every body I am working in a project involving screening of spiny lobster using 100 microsatellite groped into multiplexes.. During my research, I faced some markers that succeed in some...

22 April 2015 3,090 12 View

What is the best way to go around in solving a structural problem using the explicit method on ANSYS?

I am using ANSYS APDL to conduct a structural analysis on a structure under an axial compressive load to be able to determine its ultimate collapse strength and failure mode and I want to use an...

24 June 2014 6,711 6 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View