We are intrusted in mitochondrial cytochrome c release mechanism cause by a bacterial infection, so, how we can address this problem?
Please give more information: what kind of model system / cell line do you work with?
There are many kits available to study cytochrome-c release in apoptosis conditions.
In the attachment you will find a protocol that could be useful for you.
Cheers, Christian
Chapter Assessing Cytochrome-c Release from Mitochondria
Hi Sandeep,
maybe the paper in the attachment can help you with your question. The authors studies apoptosis initiation (e. g., cytochrome-c release) in THP1 cells.
Regards,
Christian
Article Apoptosis, in human monocytic THP.1 cells, results in the re...
Just spin down mitochondria at the high speed table centrifuge and determine the cytochrome c content spectrophotometrically.
In general, avoid using kits. Usually you have no control over what you do.
Sincerely,
Alexander Panov
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