How to analyse cell proliferation data?

02 February 2020 1 620 Report

Dear all,

I used the Click-it EdU flow cytometry assay to measure cell proliferation of my samples. However I do not know how to analyse them and interpret. How can I use the data to compare cell proliferation between two different groups?

Thanks :)

Mohammad Rafiee Monjezi

Dear Sofia at the first, gate the live cells. next, use histogram to discriminate unstained cells, stained but not stimulated cells, Positive control (which stimulated by PHA, Antibody or Ca/ionomycin ...) and your interested cells. finally compare the percentage of proliferated cell in the interested groups by proper statistic.

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