Aroona Razzaq

5 Questions 2 Answers 0 Followers

Questions related from Aroona Razzaq

How to adjust the seeding density of calculated cells suspension in 6 well plate for western Blot and Clonogenic assay?

Hello, Please guide me in the followings, I have an issue with cell seeding. When I made the cell suspension, and adding it into wells one by one, (shaking after every two/three wells), still...

06 October 2023 6,579 1 View

How to adjust DMSO concentration in working solution?

Hello, I have (100uM) stock solution in DMSO as (5mg/1850ul). I need to use 20um, 10um, 5um and 1um in working solution. How can I adjust DMSO concentration 0.1% in all of these solutions?...

10 April 2023 2,143 1 View

How to control cell number in CCK8 assay? At what density cells should be grown?

Hello, I am doing CCK8 assay for anti-cancer activity of some synthetic compounds. I have following problems in this experiment. 1. the cells number is still very high after the treatment (seems...

04 April 2023 1,558 2 View

How mass and retention time of HPLC results refers to fraction of any compound in a plant?

Hello, Dear members! please guide how mass and retention time of HPLC results refers to fraction of any compound in a plant? Profound regards!!!

08 November 2022 3,902 2 View

Why and how there is a difference in cell debris in 96 wells plate and 12 wells plate (IC50 Vs Scratch assay) with same dose concentration?

Hello comrades! I observed visible cell death (Hepg2 cells) in 96 wells plate at 400ug/mL when doing IC50 test of a certain chemical, but I didn't observed cell debris of same compound at same...

01 January 1970 7,432 0 View