LC-MS system comprised of QTrap3200 and a Shamidzu HPLC (Autosampler model is SIL-20A). The needle is not going into the well instead going in between two wells and not picking up the sample. Tried to adjust ZHoming but could not do it locally or remotely.
I am using the Shimadzu software, but now whats happening is that its injecting from the plates with a needle stroke of 40, but it's skipping one well each time. For example if it's supposed to take from A1 well it's taking from A2 and so on. I could not understand why it's doing that cause i double checked that I have the right vial position while submitting batch. Any suggestions?
Check your software whether the correct "rack" is configured. Perhaps the software thinks it is using a vial rack instead of a 96 well plate.
We control the autosampler thru the software. I use Chromeleon 7 software and I select from the Chromeleon Console> Instruments > Autosampler > Tray Control > 96 wells or 96 deep wells. After that I make sure I selected the correct tray in the software where my samples are located and under my Data console where I have my sequence, I make sure the "position" of my sample tray is correct and in agreement with the tray I selected in the Instrument software. I'm sure it must be similar with your sofware, just make sure the location of your samples and the tray you selected are the same.
I hope is just a miscommunication between your actual tray position and the one selected in the software.
Thank you so much Gerleene. I will try and make sure these things and get back to you.
Thank you so much for taking the time out to provide the detailed step by step explanation.
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