Hello,
I need help as to how to choose the right gel for SDS-PAGE. When to go for 30% acrylamide mix or for 40% acrylamide mix? Which ratio is more suitable 29:1 or 37.5:1??
As far as I know it should not make any difference at all. Depends what volume you can easier handle with. I tended to use the higher concentrated versions, so I needed to use smaller volumes.
Dear all, the gel concentration is generally selected according to the efficiency in good separation/mobility ratio. This is function of the molecular weight and its distribution of the protein in question. My Regards
30% Acrylamide
29g acrylamide
1g of N,N'-methylbisacrylamide
Dissolve in 60ml of water, adjust the volume to 100ml with water. Filtration through 0.45 micron pore size filter. Store at 40 C.
- Badges
- Science method