Binbin Lee , in cell culture experiments, the recommended concentration of 3-Methyladenine (3-MA) is 0.5-10 mM. It is not recommended to use dimethyl sulfoxide (DMSO) to prepare the stock solution. It is recommended to accurately weigh the required amount of 3-MA before the experiment, dissolve it in the culture medium, sterilize it through a 0.22 μm filter, and prepare it for use.
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