Filter paper activity (FPU) was devised by Prof. T.K Ghose for measuring overall cellulase activities. However, the most critical point of this assay was the release of exactly 2mg glucose from the Filter paper. Since the traditional Trichoderma cellulase system was deficient in beta glucosidase activity, the product released lost its linearity beyond 2 mg and reached saturation. Now the present formulations not only have enhanced beta-glucosidase activities but also high LPMO activities. Therefore how relevant it is to measure FPU activity of these samples and dose them during saccharification on FPU basis?
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