Which forward primer I should order?

I have been trying to design primers for microRNA expression. The image shows that there are 2 sets of forward primer (Tm calculated in two different manners) and one universal "reverse primer" as...

10 November 2018 5,216 0 View

How can I characterise HMEC cells?

I have a set of breast cancer cell lines and will use HMEC as a control population. Since HMECs have the tendency to show unusual characteristics along with the passages so I would like to know...

07 August 2018 6,616 0 View

Pros and cons regarding expression profiling of microRNA-SNP using breast cancer cell lines?

I have found a SNP in a microRNA gene from my association study and now want to do the expression analysis in breast cancer cell lines (sub-type specific). What are the factors should I consider...

04 May 2018 674 0 View

How can I design vector with miRNA variant (SNP) for expression analysis?

I have found a miR-SNP which is significantly associated with my breast cancer case-control cohort. Now I want to see its expression in different breast cancer cell lines to find out the best...

03 April 2018 5,145 4 View

How can I view .map & .ped files in command promt in Windows using PLINK?

I have got .bed .bim and .fam files from a database and need to see whats inside. But I don't know the command to do so. As well as I would like to view my own .ped and .map files in the command...

08 September 2017 8,758 0 View

In vitro assay to screen anti-arthritic property of drug compound?

09 October 2016 9,263 4 View

Which cell-cycle phase is the best for drug treatment?

I have used the drug as a broad term here. Specifically my focus is on those drugs which have antioxidant property. Is there really any time point at which cells will respond best to the...

07 August 2016 4,857 0 View

HUVEC starvation, time and with or without serum?

To get the best ROS generation (i am expecting 40-50% increase than that of control), how long my HUVEC should be starved and what would be the serum percentage? i am using EBM-2 (Lonza) without...

06 July 2016 9,227 5 View

HBSS, with or without Ca2+/Mg2+ for DCFDA assay?

06 July 2016 1,167 5 View

Why HUVEC is giving low amount of protein?

Trying to measure protein concentration for Lucigenin assay. But even culturing at more than 80% confluency, after centrifugation and sonication getting only 330ug/ml of protein. Hard to maintain...

05 June 2016 1,227 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View