I'd like to standard the inoculation methodology for this pathogen, but the lowest concentration that I found was 6x10^4.
Is it possible to work with a lower concentration to produce an infection in berries?
The lowest Concentration of botrytis we found in hour lab to produce an infection in strawberries is 10^6 spore/ml.
In case of Colletotrichum acutatum we found that the lowest concentration to produce an infection in strawberries is 10^4
We inoculate at 10^6 spore/ml of B.cinerea on tomato fruits and the fruits were being infected. The symptom spread fast if stored in cold storage
For tomato leaves, I've gotten good infections with a spore concentration of 1 x 10^5. The lowest I've gone for tomato fruit with consistent infections is 2 x 10^5. I use a 7% v/v white grape juice/sterile DI water solution to suspend the conidia. This increases infection efficiency on leaves.
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