How much time does it take for Luciferase Assay to work?

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Which solution reveals the microstructure and features of nickel-based alloys?

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Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

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Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

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Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

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How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Can the problems of overfitting be solved without cross validation?

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Research Gate Does it help researches to publicize their scientific work ?

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How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

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How to classify and recognize unseen image class in document sorting?

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RIA. How to measure?

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What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

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EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

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Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

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What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

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Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

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Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

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GST assay in microplate?

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What lysis buffer is compatible with Bradford assay?

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For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

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Hamadi Madhi

immediately after add the substrate. the trick consists on adding a freshly prepared substrate in a short time by using a example a dispenser instead of pipette. Moreover, it is noteworthy to know that luciferase activity is highly sensitive to temperature and has a short half-life.

Pavan Kumar

Alright, thanks for the help! I was also wondering if we could perform this test on soil samples to test it for the presence of ATP. How much amount of luciferin enzyme shall be added to let's say around 5 to 6 grams of soil?