Hello, I was looking forward to analyzing some samples using the Luciferase assay. However, I wanted to know how much time it would take for the sample to show results once the Luciferin enzyme is added to the sample?
immediately after add the substrate. the trick consists on adding a freshly prepared substrate in a short time by using a example a dispenser instead of pipette. Moreover, it is noteworthy to know that luciferase activity is highly sensitive to temperature and has a short half-life.
Alright, thanks for the help! I was also wondering if we could perform this test on soil samples to test it for the presence of ATP. How much amount of luciferin enzyme shall be added to let's say around 5 to 6 grams of soil?