How much signal do you get from Human PBMC FlowRNA negative control?

25 March 2016 1 1K Report

Hi,

I am looking at gene expression of my gene of interest in human PBMC´s with Affymetrix PRIMflow assay on Flow Cytometry. However, I did not include a negative primer in the experiment, which I regreat now. Could anyone tell me how much background staining you get from using a negative control primer. I did include FMO in the experiment, but can I trust my data? Please share your experience with me.

Saleh Alkarim

Hello

look at these links .

Hope to be useful

http://www.healthcare.uiowa.edu/corefacilities/flowcytometry/new_tech/PrimeFlowProtocol.pdf

http://www.ebioscience.com/resources/faq/rna-flow-cytometry-faq.htm

http://media.ebioscience.com/data/pdf/literature/quantigene-flowrna-user-manual.pdf

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